Binding sites; DNA binding domain; Enhancers; Transcription factors; Transcription regulation; cis-regulatory element

Systematic Determination of Transcription Factor DNA-Binding Specificities in Yeast

Understanding how genes are regulated, decoding their "regulome", is one of the main challenges of the post-genomic era. Here, we describe the in vitro method we used to associate cis-regulatory sites with cognate trans-regulators by characterizing …